p27/Kip1 Antibody (9R5B4)

Western Blot: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Analysis of extracts of various cell lines, using p27/Kip1 antibody (NBP3-15662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Rat ovary using p27/Kip1 Rabbit mAb (NBP3-15662) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 9R5B4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

p27/Kip1 Antibody (9R5B4) Summary

• Description: Novus Biologicals Rabbit p27/Kip1 Antibody (9R5B4) (NBP3-15662) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 99-198 of human p27/Kip1 (P46527). CKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDKN1B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for p27/Kip1 Antibody (9R5B4)

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN1B