p21/CIP1/CDKN1A Antibody (CIP1/823)

Images

 
Western Blot: p21/CIP1/CDKN1A Antibody (CIP1/823) [NBP2-44527] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (CIP1/823).
Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (CIP1/823) [NBP2-44527] - Analysis of HeLa cells labeling p21 with p21/CIP1/CDKN1A Antibody (CIP1/823) followed by Goat anti-Mouse IgG-CF555 (Red). ...read more
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (CIP1/823) [NBP2-44527] - Human Bladder Carcinoma stained with p21 Monoclonal Antibody (CIP1/823).
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (CIP1/823) [NBP2-44527] - Human Lung Squamous Cell Carcinoma (SCC) stained with p21 Monoclonal Antibody (CIP1/823).

Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
CIP1/823
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

p21/CIP1/CDKN1A Antibody (CIP1/823) Summary

Immunogen
Recombinant full-length human CDKN1A protein
Localization
Nuclear
Specificity
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
Application Notes
Hu-chromosome location: 6p21.31
Immunohistochemistry-Paraffin 2 - 4 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
p21/CIP1/CDKN1A Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for p21/CIP1/CDKN1A Antibody (CIP1/823)

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p21/CIP1/CDKN1A Antibody (NBP2-44527) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47783AF350
Alexa Fluor 405 NBP2-47783AF405
Alexa Fluor 488 NBP2-47783AF488
Alexa Fluor 532 NBP2-47783AF532
Alexa Fluor 594 NBP2-47783AF594
Alexa Fluor 647 NBP2-47783AF647
Alexa Fluor 700 NBP2-47783AF700
Alexa Fluor 750 NBP2-47783AF750
Allophycocyanin NBP2-47783APC
Biotin NBP2-47783B
DyLight 350 NBP2-47783UV
DyLight 405 NBP2-47783V
DyLight 488 NBP2-47783G
DyLight 550 NBP2-47783R
DyLight 594 NBP2-47783DL594
DyLight 650 NBP2-47783C
DyLight 680 NBP2-47783FR
DyLight 755 NBP2-47783IR
FITC NBP2-47783F
HRP NBP2-47783H
Janelia Fluor 549 NBP2-47783JF549
Janelia Fluor 646 NBP2-47783JF646
PE NBP2-47783PE
PerCP NBP2-47783PCP

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Bioinformatics

Gene Symbol CDKN1A
Uniprot