p19ARF Antibody

Images

 
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
Immunocytochemistry/ Immunofluorescence: p19ARF Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Immunohistochemistry-Paraffin: p19ARF Antibody [NB200-106] - IHC analysis of formalin fixed paraffin-embedded (FFPE) mouse spleen using p19ARF antibody at 10 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay ...read more
Immunocytochemistry/ Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

p19ARF Antibody Summary

Immunogen
Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF. [Swiss-Prot #P51480]
Localization
Nuclear
Predicted Species
Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDKN2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Frozen 1 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
This p19ARF antibody is useful for ELISA, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Western Blot. In WB a band can be seen at approximately 19 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
p19ARF Peptide (NB200-106PEP)
Reviewed Applications
Read 1 Review rated 3
using
NB200-106 in the following applications:

Publications
Read Publications using
NB200-106 in the following applications:

Reactivity Notes

Mouse. Immunogen sequence has 93% homology to rat, 86% homology to porcine and 80% homology to human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p19ARF Antibody

  • ARF
  • CDK4 inhibitor p16-INK4
  • CDK4IP14ARF
  • CDKN2
  • cell cycle negative regulator beta
  • CMM2P16-INK4A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • INK4
  • INK4A
  • MLMP16INK4
  • MTS-1
  • MTS1P14
  • Multiple tumor suppressor 1
  • p14
  • p14ARF
  • P16
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • P19ARF
  • TP16

Background

The ARF gene, p19ARF in mouse and p14ARF in human, has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p19ARF Antibody (NB200-106)(40)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
WB
(16)
All Applications
Filter By Species
Mouse
(23)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NB200-106 Applications Species
Xie X, Tsai Sy, Tsai Mj. COUP-TFII regulates satellite cell function and muscular dystrophy. J. Clin. Invest. Sep 12 2016 [PMID: 27617862]
Chen D, Chen Y, Forrest D, Bremner R. E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3 Cell Death Differ 2013 Apr 5 [PMID: 23558950] (WB, Mouse) WB Mouse
Wilson BG, Wang X, Shen X et al. Epigenetic antagonism between polycomb and SWI/SNF complexes during oncogenic transformation. Cancer Cell. 2010 Oct 19 [PMID: 20951942] (WB, Mouse) WB Mouse
Mills JR, Hippo Y, Robert F et al. mTORC1 promotes survival through translational control of Mcl-1. Proc Natl Acad Sci U S A 105(31):10853-8. 2008 Aug 5. [PMID: 18664580]
Wang W-H et al. Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis. J Biol Chem 283(37):25455-67. 2008 Sep 12. [PMID: 18606816]
Timmers, Cynthia et al. E2f1, E2f2, and E2f3 control E2F target expression and cellular proliferation via a p53-dependent negative feedback loop. Mol Cell Biol 27(1):65-78. 2007 Jan. [PMID: 17167174]
Iaquinta PJ, Aslanian A, Lees JA. Regulation of the Arf/p53 tumor surveillance network by E2F. Cold Spring Harb Symp Quant Biol 2005 [PMID: 16869767] (Mouse) Mouse
Klochendler-Yeivin, A et al. Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex. Mol Cell Biol 26(7):2661-74. 2006 Apr. [PMID: 16537910]
Baker, DJ et al. Early aging-associated phenotypes in Bub3/Rae1 haploinsufficient mice. J Cell Biol 172(4):529-40. 2006 Feb 13. [PMID: 16476774]
Castillo JP, Frame FM, Rogoff HA et al. Human cytomegalovirus IE1-72 activates ataxia telangiectasia mutated kinase and a p53/p21-mediated growth arrest response. J Virol 2005 Sep [PMID: 16103197]
Show All 40 Publications.

Review for p19ARF Antibody (NB200-106) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB200-106:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence p19ARF NB200-106
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reviewed by:
Anonymous
IF Mouse 05/07/2018
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Summary

ApplicationImmunofluorescence
Sample TestedAdult kidney
SpeciesMouse
LotE-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p19ARF Antibody (NB200-106). (Showing 1 - 1 of 1 FAQs).

  1. Is there a recommended antigen retrieval method for this product for use in IHC-P?
    • For NB200-106 I would recommend our standard HIER Citrate Buffer pH 6.0.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-106AF405
Alexa Fluor 488 Labeled NB200-106AF488
Alexa Fluor 647 Labeled NB200-106AF647
Alexa Fluor 700 Labeled NB200-106AF700
Biotin Labeled NB200-106B
DyLight 405 Labeled NB200-106V
DyLight 488 Labeled NB200-106G
DyLight 550 Labeled NB200-106R
DyLight 650 Labeled NB200-106C
FITC Labeled NB200-106F
HRP Labeled NB200-106H

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Bioinformatics Tool for p19ARF Antibody (NB200-106)

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Research Areas for p19ARF Antibody (NB200-106)

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Blogs on p19ARF.

You can't be without me - SNF5
The protein encoded by SNF5 is a component of the chromatin-remodeling protein complex responsible for relieving repressive chromatin structures by allowing the transcriptional machinery to access targets more effectively. SNF5 has been found to be a...  Read full blog post.

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Recent Reviews

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1
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Anonymous
05/07/2018
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CDKN2A