p18INK4c/CDKN2C Antibody - Azide and BSA Free

Western Blot: p18INK4c/CDKN2C Antibody [NBP3-03298] - Analysis of extracts from normal (control) and CDKN2C/p18-INK4C knockout (KO) HeLa cells, using p18INK4c/CDKN2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking Buffer: 3% nonfat dry milk in TBST.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

p18INK4c/CDKN2C Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human p18INK4c/CDKN2C (NP_523240.1). MAEPWGNELASAAARGDLEQLTSLLQNNVNVNAQNGFGRTALQVMKLGNPEIARRLLLRGANPDLKDRTGFAVIHDAARAGFLDTLQTLLEFQADVNIEDNEGNLPLHLAAKEGHLRVVEFLVKHTASNVGHRNHKGDTACDLARLYGRNEVVSLMQANGAGGATNLQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDKN2C
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Knockout Validated
  • Western Blot 1:500-1:2000
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for p18INK4c/CDKN2C Antibody - Azide and BSA Free

• CDK6 inhibitor p18
• CDKN2C
• CDKN6
• cyclin-dependent inhibitor
• cyclin-dependent kinase 4 inhibitor C
• Cyclin-dependent kinase 6 inhibitor
• cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
• INK4Ccyclin-dependent kinase 6 inhibitor p18
• p18
• p18INK4c
• p18-INK4C
• p18-INK6

Background

p18 INK4c is a specific inhibitor of cdk4 / cdk6 (interacts strongly with CDK6 and weakly with CDK4), and is expressed during G1 to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S phase. Highest expression levels found in skeletal muscle. p18 INK4c is also found in pancreas and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: CDKN2C