p16INK4a/CDKN2A Antibody

Images

 
Western Blot: p16INK4a/CDKN2A Antibody [NB200-106] - Analysis of p16INK4a/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody [NB200-106] - Analysis of FFPE mouse spleen using p16INK4a/CDKN2A antibody at 10 ug/mL on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes ...read more
Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

p16INK4a/CDKN2A Antibody Summary

Immunogen
Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p16INK4a. [Swiss-Prot #P51480]
Localization
Nuclear
Predicted Species
Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDKN2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Frozen 1 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
This p16INK4a antibody is useful for ELISA, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Western Blot. In WB a band can be seen at approximately 19 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Reviewed Applications
Read 1 Review rated 3
using
NB200-106 in the following applications:

Publications
Read Publications using
NB200-106 in the following applications:

Reactivity Notes

Immunogen sequence has 86% homology to porcine and 80% homology to human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p16INK4a/CDKN2A Antibody

  • ARF
  • CDK4 inhibitor p16-INK4
  • CDK4IP14ARF
  • CDKN2
  • cell cycle negative regulator beta
  • CMM2P16-INK4A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • INK4
  • INK4A
  • MLMP16INK4
  • MTS-1
  • MTS1P14
  • Multiple tumor suppressor 1
  • p14
  • p14ARF
  • P16
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • P19ARF
  • TP16

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB6798
Species: Hu
Applications: WB, ICC
NBP1-31308
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56888
Species: Hu, Mu, Rt, Ca, Eq
Applications: WB
NBP2-46085
Species: Hu
Applications: WB, IHC
H00027065-M01
Species: Hu, Rt
Applications: WB, ELISA
NBP1-82454
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82805
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00029107-M08
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
NBP1-84745
Species: Hu
Applications: IHC, IHC-P
NBP2-47560
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80616
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-13387
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90949
Species: Hu
Applications: WB, IHC, IHC-P
NB100-2736
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
AF1555
Species: Hu
Applications: WB
NBP2-67484
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB200-106
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for p16INK4a/CDKN2A Antibody (NB200-106)(41)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(16)
All Applications
Filter By Species
Mouse
(24)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using NB200-106 Applications Species
Novais EM, Diekman BO, Shapiro IM, Risbud MV. p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence Matrix Biol. Feb 24 2019 [PMID: 30811968] (IHC-P, Mouse) IHC-P Mouse
Xie X, Tsai Sy, Tsai Mj. COUP-TFII regulates satellite cell function and muscular dystrophy. J. Clin. Invest. Sep 12 2016 [PMID: 27617862]
Chen D, Chen Y, Forrest D, Bremner R. E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3 Cell Death Differ 2013 Apr 5 [PMID: 23558950] (WB, Mouse) WB Mouse
Wilson BG, Wang X, Shen X et al. Epigenetic antagonism between polycomb and SWI/SNF complexes during oncogenic transformation. Cancer Cell. 2010 Oct 19 [PMID: 20951942] (WB, Mouse) WB Mouse
Mills JR, Hippo Y, Robert F et al. mTORC1 promotes survival through translational control of Mcl-1. Proc Natl Acad Sci U S A 105(31):10853-8. 2008 Aug 5. [PMID: 18664580]
Wang W-H et al. Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis. J Biol Chem 283(37):25455-67. 2008 Sep 12. [PMID: 18606816]
Timmers, Cynthia et al. E2f1, E2f2, and E2f3 control E2F target expression and cellular proliferation via a p53-dependent negative feedback loop. Mol Cell Biol 27(1):65-78. 2007 Jan. [PMID: 17167174]
Iaquinta PJ, Aslanian A, Lees JA. Regulation of the Arf/p53 tumor surveillance network by E2F. Cold Spring Harb Symp Quant Biol 2005 [PMID: 16869767] (Mouse) Mouse
Klochendler-Yeivin, A et al. Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex. Mol Cell Biol 26(7):2661-74. 2006 Apr. [PMID: 16537910]
Baker, DJ et al. Early aging-associated phenotypes in Bub3/Rae1 haploinsufficient mice. J Cell Biol 172(4):529-40. 2006 Feb 13. [PMID: 16476774]
Show All 41 Publications.

Review for p16INK4a/CDKN2A Antibody (NB200-106) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB200-106:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence p16INK4a/CDKN2A NB200-106
Enlarge
reviewed by:
Anonymous
IF Mouse 05/07/2018
View

Summary

ApplicationImmunofluorescence
Sample TestedAdult kidney
SpeciesMouse
LotE-1

Product General Protocols

View specific protocols for p16INK4a/CDKN2A Antibody (NB200-106): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p16INK4a/CDKN2A Antibody (NB200-106). (Showing 1 - 2 of 2 FAQs).

  1. Our customer asked us about NBP1-02905, which we lately found it discontinued. While checking its related products, there seems none. Would you please help confirm if there is any replacement available?
    • With regards to your question on our CDKN2A antibodies, we do supply a wide range of products, but you haven't specified which application you are referring to. Please go through this link (cyclin-dependent kinase inhibitor 2A) and choose the product that will suit your experiment and the species you are working with. Meanwhile it is important to keep in mind that CDKN2A generates several transcript variants which differ in their first exons.
  2. Is there a recommended antigen retrieval method for this product for use in IHC-P?
    • For NB200-106 I would recommend our standard HIER Citrate Buffer pH 6.0.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-106AF405
Alexa Fluor 488 Labeled NB200-106AF488
Alexa Fluor 647 Labeled NB200-106AF647
Alexa Fluor 700 Labeled NB200-106AF700
Biotin Labeled NB200-106B
DyLight 405 Labeled NB200-106V
DyLight 488 Labeled NB200-106G
DyLight 550 Labeled NB200-106R
DyLight 650 Labeled NB200-106C
FITC Labeled NB200-106F
HRP Labeled NB200-106H

Additional p16INK4a/CDKN2A Products

Bioinformatics Tool for p16INK4a/CDKN2A Antibody (NB200-106)

Discover related pathways, diseases and genes to p16INK4a/CDKN2A Antibody (NB200-106). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for p16INK4a/CDKN2A Antibody (NB200-106)

Discover more about diseases related to p16INK4a/CDKN2A Antibody (NB200-106).
 

Pathways for p16INK4a/CDKN2A Antibody (NB200-106)

View related products by pathway.

PTMs for p16INK4a/CDKN2A Antibody (NB200-106)

Learn more about PTMs related to p16INK4a/CDKN2A Antibody (NB200-106).
 

Research Areas for p16INK4a/CDKN2A Antibody (NB200-106)

Find related products by research area.

Blogs on p16INK4a/CDKN2A

There are no specific blogs for p16INK4a/CDKN2A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

Anonymous
05/07/2018
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CDKN2A