p114RhoGEF Antibody (1972) - Azide and BSA Free

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [1972] dilution: 1:1000.

Immunohistochemistry-Paraffin: p114RhoGEF Antibody (1972) [NBP2-43546] - Mouse thymus gland. ARHGEF18 stained by ARHGEF18 antibody [1972] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [1972] dilution: 1:3000

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [1972] dilution: 1:1000

Immunohistochemistry-Paraffin: p114RhoGEF Antibody (1972) [NBP2-43546] - Rat lymph node. ARHGEF18 stained by ARHGEF18 antibody [1972] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARHGEF18 antibody [1972] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 1972
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

p114RhoGEF Antibody (1972) - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human p114RhoGEF. The exact sequence is proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARHGEF18
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 30948425).
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for p114RhoGEF Antibody (1972) - Azide and BSA Free

• 114 kDa Rho-specific guanine nucleotide exchange factor
• EC 3.4.24
• EC 3.6.1
• KIAA0521rho guanine nucleotide exchange factor 18
• MGC15913
• p114RhoGEF
• P114-RhoGEF
• P114-RHO-GEF
• Rho/Rac guanine nucleotide exchange factor (GEF) 18
• Rho/Rac guanine nucleotide exchange factor 18
• Rho-specific guanine nucleotide exchange factor p114
• SA-RhoGEF
• Septin-associated RhoGEF

Background

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p114RhoGEF Antibody (NBP2-43546)(1)

Publication using NBP2-43546

• Juettner, VV;Kruse, K;Dan, A;Vu, VH;Khan, Y;Le, J;Leckband, D;Komarova, Y;Malik, AB; VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism J. Cell Biol. 2019-04-04 [PMID: 30948425] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: ARHGEF18