P-Cadherin Antibody (106020) [Unconjugated]

A431 human epithelial carcinoma cell line was stained with Rat Anti-Human/Mouse P-Cadherin Monoclonal Antibody (Catalog # MAB761, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu
• Applications: WB, Flow, CyTOF-ready
• Clone: 106020
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

P-Cadherin Antibody (106020) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse P-Cadherin
  Glu100-Gly647
  Accession # Q8BSL6
• Specificity: Detects mouse P-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) N-Cadherin, recombinant mouse (rm) VE-Cadherin, rhCadherin-8, or rhCadherin-17 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CDH3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Cadherin Antibody (106020) [Unconjugated]

• CAD3
• cadherin 3, P-cadherin (placental)
• cadherin 3, type 1, P-cadherin (placental)
• Cadherin-3
• CDH3
• CDHP
• CDHPcalcium-dependent adhesion protein, placental
• HJMD
• PCAD
• PCadherin
• P-Cadherin
• PCADP-cadherin
• Placental cadherin

Background

Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules, designated CDH3. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region.

1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
2. Overduin, M. et al. (1995) Science 267:386.
3. Takeichi, M. (1991) Science 251:1451.
4. Nose, A. et al. (1987) EMBO J. 6:3655.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Cadherin Antibody (MAB761)(5)

Publications using MAB761

• Dejan Maglic, Karin Schlegelmilch, Antonella FM Dost, Riccardo Panero, Michael T Dill, Raffaele A Calogero et al. YAP‐TEAD signaling promotes basal cell carcinoma development via a c‐JUN/AP1 axis The EMBO Journal 9/3/2018 [PMID: 30037824]
• JE Hoefert, GA Bjerke, D Wang, R Yi The microRNA-200 family coordinately regulates cell adhesion and proliferation in hair morphogenesis J. Cell Biol., 2018-03-30;0(0):. 3/30/2018 [PMID: 29602800]
• Li S, Huang P, Yang A, Tarng D, Yang W, Lin C, Chen J, Schmid-Schonbein G, Lin S Matrix metalloproteinase-9 deficiency attenuates diabetic nephropathy by modulation of podocyte functions and dedifferentiation. Kidney Int, 2014-03-26;86(2):358-69. 2014-03-26 [PMID: 24670409] (ICC, IHC-Fr, Western Blot, Mouse)
• Gozde Yucel, Banu Altindag, Natalia Gomez-Ospina, Anshul Rana, Georgia Panagiotakos, Maria Fernanda Lara et al. State-dependent signaling by Cav1.2 regulates hair follicle stem cell function Genes & Development 6/1/2013 [PMID: 23752588]
• Tinkle CL, Pasolli HA, Stokes N, Fuchs E New insights into cadherin function in epidermal sheet formation and maintenance of tissue integrity. Proc. Natl. Acad. Sci. U.S.A., 2008-09-22;105(40):15405-10. 2008-09-22 [PMID: 18809908] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CDH3
• Uniprot:
  • Q8BSL6()