OX40 Ligand/TNFSF4 Antibody (182609) [HRP]

• Reactivity: Mu
• Applications: WB, ELISA, ELISA(Cap), ELISA(Det)
• Clone: 182609
• Clonality: Monoclonal
• Host: Rat
• Conjugate: HRP

OX40 Ligand/TNFSF4 Antibody (182609) [HRP] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse OX40 Ligand/TNFSF4
  Gln49-Leu198
  Accession # P43488
• Specificity: Detects mouse OX40 Ligand/TNFSF4 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) OX40, recombinant human (rh) OX40 Ligand, rmFas Ligand, rmLIGHT, or rhLIGHT is observed. In Western blots, 100% cross-reactivity with rmTNF‑ alpha is observed, 15‑30% with rmTRANCE and rmTRAIL is observed, and no cross-reactivity with rmBAFF, rmEDA, rmGITR Ligand, rmLIGHT, rmTWEAK, rmFas Ligand, rhAPRIL, or rhOX40 Ligand is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFSF4
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for OX40 Ligand/TNFSF4 Antibody (182609) [HRP]

• CD134 ligand
• CD134L
• CD252 antigen
• CD252
• Glycoprotein Gp34
• gp34
• OX40 antigen ligand
• OX40 Ligand
• OX40L
• OX-40L
• OX4OL
• TAX transcriptionally-activated glycoprotein 1
• tax-transcriptionally activated glycoprotein 1 (34kD)
• tax-transcriptionally activated glycoprotein 1, 34kD
• TNFSF4
• tumor necrosis factor (ligand) superfamily, member 4
• tumor necrosis factor ligand superfamily member 4
• TXGP1

Background

CD252 is a 35 kD member of the TNF superfamily also known as OX40L and CD134L. The OX40 ligand is expressed on activated B cells and antigen presenting cells. OX40 ligand interacts with OX40 antigen (CD134) expressed predominantly on activated T cells to increase proliferation and IL-2 production, and to enhance proliferation and immunoglobulin secretion in activated B cells. The RM134L antibody can inhibit OX40/OX40 ligand interactions and block the costimulatory activity of OX40L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF4