OX40 Ligand/TNFSF4 Antibody (159410) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA
• Clone: 159410
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

OX40 Ligand/TNFSF4 Antibody (159410) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human OX40 Ligand/TNFRSF4
  Gln51-Leu183
  Accession # P23510
• Specificity: Detects human OX40 Ligand/TNFSF4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OX40 Ligand/TNFSF4 Antibody (159410) [Unconjugated]

• CD134 ligand
• CD134L
• CD252 antigen
• CD252
• Glycoprotein Gp34
• gp34
• OX40 antigen ligand
• OX40 Ligand
• OX40L
• OX-40L
• OX4OL
• TAX transcriptionally-activated glycoprotein 1
• tax-transcriptionally activated glycoprotein 1 (34kD)
• tax-transcriptionally activated glycoprotein 1, 34kD
• TNFSF4
• tumor necrosis factor (ligand) superfamily, member 4
• tumor necrosis factor ligand superfamily member 4
• TXGP1

Background

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1 - 23) and a carboxy-terminal extracellular domain (aa 51 - 183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4⁺ T cells. OX40 Ligand is one of the co-stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4-1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4⁺ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

1. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757.
2. Baum, P.R. et al. (1994) EMBO J. 13:3992.
3. Al-Shamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
4. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P23510()