Otubain-1 Antibody

Western Blot: Otubain-1 Antibody [NBP1-20957] - Staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Otubain-1 Antibody Summary

• Immunogen: Peptide with sequence C-YKEYAEDDNIYQQK corresponding to internal region according to NP_060140.2.
• Predicted Species: Human (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OTUB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 30k Da band observed in mouse brain lysates (calculated MW of 31.3 kDa according to Human NP_060140.2 and Mouse NP_598911.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Otubain-1 Antibody

• Deubiquitinating enzyme OTUB1
• EC 3.1.2
• EC 3.4.19.12
• FLJ20113
• FLJ40710
• hOTU1
• MGC111158
• OTB1
• OTB1MGC4584
• OTU domain, ubiquitin aldehyde binding 1
• OTU domain-containing ubiquitin aldehyde-binding protein 1
• OTU1
• OTU1OTU-domain Ubal-binding 1
• OTUB1
• Otubain1
• Otubain-1
• ubiquitin thioesterase OTUB1
• ubiquitin-specific protease otubain 1
• Ubiquitin-specific-processing protease OTUB1

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Otubain-1 Antibody (NBP1-20957)(1)

Publication using NBP1-20957

• Juris SJ, Shah K, Shokat K et al. Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry. FEBS Lett 2006-01-09 [PMID: 16364312]

Bioinformatics

• Gene Symbol: OTUB1
• Entrez:
  • 55611(Human)
  • 107260(Mouse)
  • 293705(Rat)
• Uniprot:
  • NP_060140.2(Human)