Otubain-1 Antibody (1C12) - Azide and BSA Free

Western Blot: Otubain-1 Antibody (1C12) [H00055611-M09] - Analysis of OTUB1 expression in Hela S3 NE (Cat # L013V3).

ELISA: Otubain-1 Antibody (1C12) [H00055611-M09] - Detection limit for recombinant GST tagged OTUB1 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Otubain-1 Antibody (1C12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Otubain-1 Antibody (1C12) - Azide and BSA Free (H00055611-M09) is a monoclonal antibody validated for use in WB and ELISA. Anti-Otubain-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: OTUB1 (AAH07519, 1 a.a. ~ 271 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAEEPQQQKQEPLGSDSEGVNCLAYDEANMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK
• Specificity: OTUB1 - OTU domain, ubiquitin aldehyde binding 1 (1C12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTUB1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Otubain-1 Antibody (1C12) - Azide and BSA Free

• Deubiquitinating enzyme OTUB1
• EC 3.1.2
• EC 3.4.19.12
• FLJ20113
• FLJ40710
• hOTU1
• MGC111158
• OTB1
• OTB1MGC4584
• OTU domain, ubiquitin aldehyde binding 1
• OTU domain-containing ubiquitin aldehyde-binding protein 1
• OTU1
• OTU1OTU-domain Ubal-binding 1
• OTUB1
• Otubain1
• Otubain-1
• ubiquitin thioesterase OTUB1
• ubiquitin-specific protease otubain 1
• Ubiquitin-specific-processing protease OTUB1

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Otubain-1 Antibody (H00055611-M09)(1)

Publication using H00055611-M09

• Edelmann MJ, Kramer HB, Altun M, Kessler BM. Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion. FEBS J. 2010-06-01 [PMID: 20553488]

Bioinformatics

• Gene Symbol: OTUB1
• Entrez:
  • 55611(Human)
• Uniprot:
  • AAH07519(Human)