Osteoadherin/OSAD/OMD Antibody (806001)

Osteoadherin/OSAD was detected in immersion fixed human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human Osteoadherin/OSAD Monoclonal Antibody (Catalog # MAB2884) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 806001
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Osteoadherin/OSAD/OMD Antibody (806001) Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human Osteoadherin/OSAD
  Gln21-Gln421
  Accession # Q99983
• Specificity: Detects human Osteoadherin/OSAD in ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: OMD
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteoadherin/OSAD/OMD Antibody (806001)

• Keratan sulfate proteoglycan osteomodulin
• KSPG osteomodulin
• OMD
• OSAD
• osteoadherin proteoglycan
• Osteoadherin
• osteomodulin
• SLRR2C
• SLRR2Costeoadherin

Background

Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately 20-30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix (1, 2). The human OSAD cDNA encodes a 421 aa precursor that contains a 20 aa signal sequence and eleven tandem leucine rich repeats (3). Human OSAD shares 80-84% aa sequence identity with bovine, mouse, and rat OSAD. Human OSAD shares 32-35% aa sequence identity with human class II SLRPs Fibromodulin, Keratocan, Lumican, and PRELP. Bovine, mouse, and rat OSAD are expressed as 60-85 kDa molecules, although the amino acid sequence for each predicts a size of 46-47 kDa. The primary difference is due to the presence of extensive N-linked glycosylation that can also vary between tissues of the same species (4, 5). Human OSAD is expressed as an even larger 110 kDa molecule in teeth (6). OSAD contains eight sulfated tyrosine residues (4, 7) and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C-terminal acidic domain (3). OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon (3, 4, 8). In dental tissue, OSAD is expressed by odontoblasts and ameloblasts (5, 9-11) and is involved in the mineralization of bone and teeth (5, 11, 12). OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3 (4, 6).

1. Matsushima, N. et al. (2000) Proteins 38:210. 
2. Kobe, B. and A.V. Kajava (2001) Curr. Opin. Struct. Biol. 11:725. 
3. Sommarin, Y. et al. (1998) J. Biol. Chem. 273:16723. 
4. Wendel, M. et al. (1998) J. Cell Biol. 141:839. 
5. Hultenby, P.U. et al. (2003) Eur. J. Oral Sci. 111:128. 
6. Lucchini, M. et al. (2004) J. Dent. Res. 83:552. 
7. Onnerfjord, P. et al. (2004) J. Biol. Chem. 279:26
8. Shen, Z. et al. (1999) Matrix Biol. 18:533.
9. Buchaille, R. et al. (2000) Bone 27:265.
10. Buchaille, R. et al. (2000) Matrix Biol. 19:421.
11. Couble, M.L. et al. (2004) Histochem. Cell Biol. 121:47.
12. Ramstad, V.E. et al. (2003) Calcif. Tissue Int. 72:57.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OMD
• Uniprot:
  • Q99983()