Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mouse mAb against PANC1 (1) and PC-3 (2) cell lysate.

Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using GPNMB mouse mAb with DAB staining.

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mAb against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)

Western Blot: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Western blot analysis using GPNMB mAb against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPNMB mouse mAb with DAB staining.

ELISA: Osteoactivin/GPNMB Antibody (7C10E5) [NBP2-37351] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 7C10E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Osteoactivin/GPNMB (AA: 31-260) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPNMB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Osteoactivin/GPNMB Antibody (7C10E5) - BSA Free

• DC-HIL
• glycoprotein (transmembrane) nmb
• glycoprotein NMB
• GPNMB
• HGFIN
• HGFINglycoprotein nmb-like protein
• NMBosteoactivin
• Osteoactivin
• Transmembrane glycoprotein HGFIN
• transmembrane glycoprotein NMB

Background

The GPNMB gene codes for a type I transmembrane glycoprotein NBM. The GPNMB gene is present in lowly, but not highly, metastic human melanoma cell lines, therefore, it demonstrates involvement in development, proliferation, and differentiation of tissues. Isoform 1 is 572 amino acids long at a mass of nearly 64 kDA and isoform 2 exists at 560 amino acids long at approximately 62 kDA. This gene has been known to interact with various genes such as SMAD4, PLA2G4A, and ITSN2. GPNMB has been researched in its role in retinitis, lung cancer, melanoma, glioblastoma, glaucoma, and breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPNMB