OSBPL11 Antibody

Western Blot: OSBPL11 Antibody [NB100-1337] - (1ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

OSBPL11 Antibody Summary

• Immunogen: Peptide with sequence C-KPLWKIIPTTQPAE corresponding to C-Terminus according to NP_073613.2.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OSBPL11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 2 ug/ml
• Application Notes: WB: Approx. 85-90 kDa band observed in human brain lysates (predicted MW of 86 kDa band according to NP_073613). A minor band of unknown identity was also consistently observed at 75 kDa.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  OSBPL11 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22241858).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OSBPL11 Antibody

• FLJ13012
• FLJ13164
• ORP11OSBP-related protein 11
• ORP-11TCCCIA00292
• OSBP12
• oxysterol binding protein-like 11
• oxysterol-binding protein-related protein 11

Background

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OSBPL11 Antibody (NB100-1337)(2)

Publications using NB100-1337

• Storey SM, McIntosh AL, Huang H et al. Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes Am J Physiol Gastrointest Liver Physiol 2012-04-15 [PMID: 22241858] (WB, Mouse)
• Lehto M, Laitinen S, Chinetti G et al. The OSBP-related protein family in humans. J Lipid Res 2001-08-01 [PMID: 11483621]

Bioinformatics

• Gene Symbol: OSBPL11
• Entrez:
  • 114885(Human)
• Uniprot:
  • NP_073613(Human)