OSBPL11 Antibody (OTI6A6)

Western Blot: OSBPL11 Antibody (6A6) [NBP2-03327] Analysis of extracts (35ug) from 9 different cell lines by using anti-OSBPL11 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: OSBPL11 Antibody (6A6) [NBP2-03327] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBPL11.

Flow Cytometry: OSBPL11 Antibody (6A6) [NBP2-03327] - Analysis of Jurkat cells, using anti-OSBPL11 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: OSBPL11 Antibody (6A6) [NBP2-03327] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11.

Flow Cytometry: OSBPL11 Antibody (6A6) [NBP2-03327] - Analysis of Hela cells, using anti-OSBPL11 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI6A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.78 mg/ml

OSBPL11 Antibody (OTI6A6) Summary

• Immunogen: Full length human recombinant protein of human OSBPL11 (NP_073613) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: OSBPL11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 83.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.78 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OSBPL11 Antibody (OTI6A6)

• FLJ13012
• FLJ13164
• ORP11OSBP-related protein 11
• ORP-11TCCCIA00292
• OSBP12
• oxysterol binding protein-like 11
• oxysterol-binding protein-related protein 11

Background

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OSBPL11
• Entrez:
  • 114885(Human)