OSBP1 Antibody

Western Blot: OSBP1 Antibody [NB100-1500] - Analysis of Human Placenta lysate (35ug protein in RIPA buffer) using NB100-1500 (0.1ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

OSBP1 Antibody Summary

• Description: Novus Biologicals Goat OSBP1 Antibody (NB100-1500) is a polyclonal antibody validated for use in WB, ELISA and IP. Anti-OSBP1 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KHQLEETKKEKRTRIP corresponding to internal region according to NP_002547.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OSBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 85 kDa band observed in human placenta lysates (calculated MW of 89.4 kDa band according to NP_002547). IP reactivity reported in (PMID: 18230613).
• Theoretical MW: 89 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  OSBP1 Overexpression Lysate

• Control Peptide:

  OSBP1 Peptide (NB100-1500PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OSBP1 Antibody

• OSBP1oxysterol-binding protein 1
• oxysterol binding protein

Background

Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OSBP1 Antibody (NB100-1500)(5)

Publications using NB100-1500

• Storey SM, McIntosh AL, Huang H et al. Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes Am J Physiol Gastrointest Liver Physiol 2012-04-15 [PMID: 22241858]
• Amako Y, Sarkeshik A, Hotta H et al. Role of Oxysterol Binding Protein in Hepatitis C Virus infection. J Virol :JVI00958-00909. 2009-01-01 [PMID: 19570870]
• Takeshita M, Ishida Y-i, Akamatsu E et al. Proanthocyanidin from Blueberry Leaves Suppresses Expression of Subgenomic Hepatitis C Virus RNA. J Biol Chem;284(32):21165-21176. 2009-01-01 [PMID: 19531480]
• Romeo GR, Kazlauskas A. Oxysterol Diabetes Activate STAT3 Control Endothelial Expression of Profilin-1 via OSBP1. J Biol Chem;283(15):9595-605. 2008-04-11 [PMID: 18230613]
• Wang PY, Weng J, Anderson RG. OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation. Science 2005-03-04 [PMID: 15746430]

Bioinformatics

• Gene Symbol: OSBP
• Entrez:
  • 5007(Human)
• Uniprot:
  • P22059(Human)