OSBP1 Overexpression Lysate (Native)

Images

 
Western Blot: OSBP1 Overexpression Lysate (Adult Normal) [NBL1-13984] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OSBP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

OSBP1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204250 and based on accession number NM_002556. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens oxysterol binding protein (OSBP), mRNA.
Gene
OSBP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
89.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for OSBP1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for OSBP1 Overexpression Lysate (Native)

  • OSBP1oxysterol-binding protein 1
  • oxysterol binding protein

Background

Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-02300
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP1-85681
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83132
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-81388
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-92236
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82968
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1331
Species: Hu
Applications: WB, IHC-P, PEP-ELISA
NB400-157
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western
NBP1-52084
Species: Hu, Mu, Rt
Applications: WB, IHC-P, PEP-ELISA
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, RIA
NB400-105
Species: Hu, Mu, Rt, Po, Ca, Ch, Ha, Md
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, GS
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
NBP1-87789
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-85954
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-81403
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2448
Species: Hu
Applications: WB, PEP-ELISA
NBP1-32870
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP

Publications for OSBP1 Lysate (NBL1-13984) (0)

There are no publications for OSBP1 Lysate (NBL1-13984).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for OSBP1 Lysate (NBL1-13984) (0)

There are no reviews for OSBP1 Lysate (NBL1-13984). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for OSBP1 Lysate (NBL1-13984) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional OSBP1 Products

Bioinformatics Tool for OSBP1 Lysate (NBL1-13984)

Discover related pathways, diseases and genes to OSBP1 Lysate (NBL1-13984). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for OSBP1 Lysate (NBL1-13984)

Discover more about diseases related to OSBP1 Lysate (NBL1-13984).
 

Pathways for OSBP1 Lysate (NBL1-13984)

View related products by pathway.

PTMs for OSBP1 Lysate (NBL1-13984)

Learn more about PTMs related to OSBP1 Lysate (NBL1-13984).

Blogs on OSBP1

There are no specific blogs for OSBP1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

ABCA1 Antibody
NB400-105

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our OSBP1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol OSBP