OSBP1 Antibody

Western Blot: OSBP1 Antibody [NB100-1329] - (2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

OSBP1 Antibody Summary

• Immunogen: Peptide with sequence CKEKQDWSSCPDIF corresponding to C-Terminus according to NP_002547.1.
• Localization: Cytoplasmic and Golgi Apparatus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OSBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 100 kDa band observed in human liver lysates (calculated MW of 89.4 kDa band according to NP_002547.1). Use in Immunoprecipitation reported in scientific literature (PMID: 18230613)
• Control:

  OSBP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OSBP1 Antibody

• OSBP1oxysterol-binding protein 1
• oxysterol binding protein

Background

Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OSBP1 Antibody (NB100-1329)(2)

Publications using NB100-1329

• Romeo GR, Kazlauskas A. Oxysterol Diabetes Activate STAT3 Control Endothelial Expression of Profilin-1 via OSBP1. J Biol Chem;283(15):9595-605. 2008-04-11 [PMID: 18230613] (IP)
• Moreira EF, Jaworski C, Li A, Rodriguez IR. Molecular and biochemical characterization of a novel oxysterol-binding protein (OSBP2) highly expressed in retina. J Biol Chem 2001-05-25 [PMID: 11278871]

Bioinformatics

• Gene Symbol: OSBP
• Entrez:
  • 5007(Human)
• Uniprot:
  • NP_002547(Human)