Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free

Analysis in human liver and tonsil tissues using NBP3-44105 antibody. Corresponding Ornithine Carbamoyltransferase RNA-seq data are presented for the same tissues.

Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Analysis in human liver tissue.

Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.

Staining of RH-30 cells using the Anti-Ornithine Carbamoyltransferase monoclonal antibody) .

Staining of human tonsil shows no positivity in lymphoid cells as expected.

• Reactivity: Hu, Pm-Cm
• Applications: WB, ICC/IF, IHC
• Clone: CL4045
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free (NBP2-59038) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P00480, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:10000 - 1:20000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Reactivity Notes

Use in Cynomolgus Monkey reported in scientific literature (PMID:35211641). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free

• EC 2.1.3
• EC 2.1.3.3
• MGC129967
• MGC129968
• MGC138856
• OCTD
• Ornithine Carbamoyltransferase
• ornithine carbamoyltransferase, mitochondrial
• Ornithine Transcarbamylase
• OTC
• OTCase

Background

This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTC