![Western Blot: Orexin B/Hypocretin-2 Antibody [NBP2-42905] - Analysis of Rat tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with Orexin B antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize2/Orexin-B-Hypocretin-2-Antibody-Western-Blot-NBP2-42905-img0002.jpg "Western Blot: Orexin B/Hypocretin-2 Antibody [NBP2-42905] - Analysis of Rat tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with Orexin B antibody at a dilution of 1:1000.")
| Reactivity | Mu, Rt, Hu, RMSpecies Glossary |
| Applications | WB, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Orexin B. The exact sequence is proprietary. |
| Predicted Species | Human (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HCRT |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 13 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Immunogen affinity purified |
![Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535] - Low magnification images showing the double staining pattern of NHE1 with neuropeptide NPY. Scale bar: 100um. Insets: High magnification images showing individual cells with double staining. Scale bar: 10um. Asterisks mark Hoechst-stained nuclei. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-42872-w), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Neuropeptide-Y-Antibody-Immunohistochemistry-NBP1-46535-img0001.jpg)
![Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/images2/POMC-Antibody-Western-Blot-NB100-1533-img0005.jpg)
![Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg)
![Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.](http://images.novusbio.com/images2/POMC-Antibody-Immunohistochemistry-NB100-1533-img0007.jpg)
![Immunohistochemistry: PMCH Antibody [NBP2-57697] - Staining of mouse brain shows strong positivity in of neurons in the hypothalamus.](http://images.novusbio.com/images2/PMCH-Antibody-Immunohistochemistry-NBP2-57697-img0001.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-57697] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/images2/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-57697-img0002.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-57697] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-57697-img0003.jpg)
![Western Blot: Orexin R1/HCRTR1 Antibody [NBP2-95106] - Analysis of extracts of C6 cells, using HCRTR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/images2/Orexin-R1-HCRTR1-Antibody-Western-Blot-NBP2-95106-img0001.jpg)
![Immunohistochemistry: Orexin R1/HCRTR1 Antibody [NBP2-95106] - Analysis of mouse brain using HCRTR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/Orexin-R1-HCRTR1-Antibody-Immunohistochemistry-NBP2-95106-img0002.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Orexin B/Hypocretin-2 Antibody (NBP2-42905)Discover more about diseases related to Orexin B/Hypocretin-2 Antibody (NBP2-42905).
| Pathways for Orexin B/Hypocretin-2 Antibody (NBP2-42905)View related products by pathway.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | HCRT |
![Western Blot: Orexin B/Hypocretin-2 Antibody [NBP2-42905] - Analysis of mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with Orexin B antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize2/Orexin-B-Hypocretin-2-Antibody-Western-Blot-NBP2-42905-img0001.jpg "Western Blot: Orexin B/Hypocretin-2 Antibody [NBP2-42905] - Analysis of mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with Orexin B antibody at a dilution of 1:1000.")
![Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/c-Fos-Antibody-(2H2)-Knockout-Validated-NBP2-50037-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.](http://images.novusbio.com/images2/c-Fos-Antibody-(2H2)-Immunocytochemistry-Immunofluorescence-NBP2-50037-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).](http://images.novusbio.com/images2/c-Fos-Antibody-(2H2)-Immunocytochemistry-Immunofluorescence-NBP2-50037-img0001.jpg)
![Western Blot: Corticotropin Releasing Factor Antibody (2B11) [H00001392-M02] - Analysis of CRH expression in transfected 293T cell line by CRH monoclonal antibody (M02), clone 2B11.Lane 1: CRH transfected lysate(21.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/Corticotropin-Releasing-Factor-Antibody-(2B11)-Western-Blot-H00001392-M02-img0002.jpg)
![Sandwich ELISA: Corticotropin Releasing Factor Antibody (2B11) [H00001392-M02] - Detection limit for recombinant GST tagged CRH is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/images2/Corticotropin-Releasing-Factor-Antibody-(2B11)-Sandwich-ELISA-H00001392-M02-img0001.jpg)
![Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 expression in transfected 293T cell line by HCRTR2 monoclonal antibody (M01), clone 1E3.Lane 1: HCRTR2 transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/Orexin-R2-HCRTR2-Antibody-(1E3)-Western-Blot-H00003062-M01-img0006.jpg)
![ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Detection limit for recombinant GST tagged HCRTR2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/images2/Orexin-R2-HCRTR2-Antibody-(1E3)-ELISA-H00003062-M01-img0005.jpg)
![Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 over-expressed 293 cell line, cotransfected with HCRTR2 Validated Chimera RNAi ( Cat # H00003062-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HCRTR2 monoclonal antibody (M01), clone 1E3 (Cat # H00003062-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/images2/Orexin-R2-HCRTR2-Antibody-(1E3)-Western-Blot-H00003062-M01-img0004.jpg)
![Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p<0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY licence.](http://images.novusbio.com/images2/PLOD1-Antibody-Western-Blot-NBP2-38770-img0005.jpg)
![Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-251 MG and human cell line RT-4.](http://images.novusbio.com/images2/PLOD1-Antibody-Western-Blot-NBP2-38770-img0006.jpg)
![Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/images2/PLOD1-Antibody-Western-Blot-NBP2-38770-img0007.jpg)
![Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in human cell line A-431.](http://images.novusbio.com/images2/LAMC2-Antibody-(CL2980)-Western-Blot-NBP2-42388-img0016.jpg)
![Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/images2/LAMC2-Antibody-(CL2980)-Western-Blot-NBP2-42388-img0017.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human skin shows moderate positivity in basement membrane of epidermis.](http://images.novusbio.com/images2/LAMC2-Antibody-(CL2980)-Immunohistochemistry-Paraffin-NBP2-42388-img0018.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")