Orexin R2/HCRTR2 Antibody (1E3.)

Images

 
Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 expression in transfected 293T cell line by HCRTR2 monoclonal antibody (M01), clone 1E3.Lane 1: HCRTR2 transfected lysate(50.7 KDa).Lane ...read more
Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 over-expressed 293 cell line, cotransfected with HCRTR2 Validated Chimera RNAi ( Cat # H00003062-R01V ) (Lane 2) or non-transfected ...read more
Sandwich ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Detection limit for recombinant GST tagged HCRTR2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, RNAi
Clone
1E3.
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Orexin R2/HCRTR2 Antibody (1E3.) Summary

Immunogen
HCRTR2 (NP_001517, 1 a.a. - 54 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYE
Localization
Isoform 1 and isoform 4: Cell membrane; single pass type I membrane protein. Isoform 2 and isoform 3: Secreted protein.
Specificity
HCRTR2 - hypocretin (orexin) receptor 2
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HCRTR2
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • RNA Inhibition
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Reviewed Applications
Read 1 Review rated 5
using
H00003062-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Ascites

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Orexin R2/HCRTR2 Antibody (1E3.)

  • HCRTR2
  • hypocretin (orexin) receptor 2
  • Hypocretin receptor type 2
  • Orexin R2
  • Orexin Receptor 2
  • orexin receptor type 2
  • OrexinR2
  • OX2R
  • Ox-2-R
  • ox2-R

Background

HCRTR2 is a G-protein coupled receptor expressed exclusively in the brain. It has 64% identity with HCRTR1. HCRTR2 binds both orexin A and orexin B neuropeptides. HCRTR2 is involved in the central feedback mechanism that regulates feeding behaviour.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Orexin R2/HCRTR2 Antibody (H00003062-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00003062-M01:
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(1)
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Images Ratings Applications Species Date Details
Western Blot Orexin R2/HCRTR2 H00003062-M01
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reviewed by:
Anonymous
WB Human 06/08/2011
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Summary

ApplicationWestern Blot
Sample TestedH00003062-Q01 and H00003062-B01, Sample Amount:.01,.025,.05, 0.1, 0.2 ug
SpeciesHuman
CommentsLinear relation for Ox2R monoclonal antibody immuno-recognition of human orexin receptor 2 (Swiss-Prot NP_001517) peptide. Recombinant receptor peptide corresponds to amino acids 1-55 at the N-terminus (MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWRE) tagged with a recombinant glutathione S-transferase (GST) fusion protein with a molecular weight of 35-45 kDa. The amino acid sequence for human orexin receptor 2 sequence is 94% homologous to rat. R squared value, 0.9678. I used the Millipore Snap ID system for blocking, washing and secondary incubations. I listed the secondary antibody dilution as 1:50000 but keep in mind this is 0.25 ug of antibody in 10 ml of blocking buffer. If you are using the Snap ID system then the dilution would be 1:6000 (0.25 ug in 1.5 ml. The transfer system used was BioRad's semi dry at 20 V for 30 min, transfer buffer was BioRad'S TG buffer (20% methanol). Membrane used was PVDF (Thermo Scientific).

Blocking

Blocking DetailsBuffer: SuperBlock T20 (TBS), proprietary protein formulation in TBS, pH7.4 w/ 0.05% Tween-20 & Kathon, Time/Temp: 3min at 25C

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: SuperBlock T20 (TBS), Incubation Time/Temp: overnight at 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Novus rabbit polyclonal anti-mouse HRP, Secondary Ab Dilution Ratio: 1:50000
Secondary Manufacturer Cat#Novus NB720-H

Details

Detection NotesDetection Method: HRP-West Pico (Thermo Scientific), Exposure Time: 30 seconds, Specific Bands: 35-45 kDa

Comments

CommentsLinear relation for Ox2R monoclonal antibody immuno-recognition of human orexin receptor 2 (Swiss-Prot NP_001517) peptide. Recombinant receptor peptide corresponds to amino acids 1-55 at the N-terminus (MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWRE) tagged with a recombinant glutathione S-transferase (GST) fusion protein with a molecular weight of 35-45 kDa. The amino acid sequence for human orexin receptor 2 sequence is 94% homologous to rat. R squared value, 0.9678. I used the Millipore Snap ID system for blocking, washing and secondary incubations. I listed the secondary antibody dilution as 1:50000 but keep in mind this is 0.25 ug of antibody in 10 ml of blocking buffer. If you are using the Snap ID system then the dilution would be 1:6000 (0.25 ug in 1.5 ml. The transfer system used was BioRad's semi dry at 20 V for 30 min, transfer buffer was BioRad'S TG buffer (20% methanol). Membrane used was PVDF (Thermo Scientific).

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Anonymous
06/08/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol HCRTR2
Entrez
OMIM
Uniprot