Rat Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

ELISA: Rat Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) [NBP2-80232] - Samples were spiked with high concentrations of Rat Orexin A/Hypocretin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) [NBP2-80232] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 62.5-4000 pg/mL
• Sensitivity: 37.5 pg/mL

Rat Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat OXA in samples. No significant cross-reactivity or interference between Rat OXA and analogues was observed.
• Standard Curve Range: 62.5-4000 pg/mL
• Sensitivity: 37.5 pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 5.39%
• Intra-Assay: CV% < 4.44%
• Spike Recovery: 86-100%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HCRT

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

• HCRT
• Hcrt1
• hypocretin (orexin) neuropeptide precursor
• Hypocretin1
• Hypocretin-1
• NRCLP1
• Orexin A
• orexin
• OXHcrt
• PPORX
• PPOXHypocretin

Background

FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity (By similarity). SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum (By similarity). Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses (By similarity). SIMILARITY: Belongs to the orexin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Orexin A/Hypocretin-1 ELISA Kit (NBP2-80232). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HCRT