Orai1 Antibody

Western Blot: Orai1 Antibody [NBP2-76955] - Western blot analysis of Orai1 on PC-3M cell lysate using anti-Orai1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Orai1 Antibody [NBP2-76955] - ICC staining Orai1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Orai1 Antibody [NBP2-76955] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Orai1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Orai1 Antibody [NBP2-76955] - Flow cytometric analysis of MCF-7 cells with Orai1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Orai1 Antibody [NBP2-76955] - ICC staining Orai1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Orai1 Antibody [NBP2-76955] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- Orai1 antibody. Counter stained with hematoxylin.

Product Discontinued

Orai1 Antibody Summary

• Immunogen: Synthetic peptide within mouse Orai1 aa 181-230 / 304. (SwissProt: Q96D31 Human; SwissProt: Q8BWG9 Mouse)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ORAI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Orai1 Antibody

• CRACM1
• CRACM1ORAT1
• FLJ14466
• ORAI calcium release-activated calcium modulator 1
• Orai1
• ORAT1
• Protein orai-1
• TMEM142A
• TMEM142Acalcium release-activated calcium modulator 1
• Transmembrane protein 142Acalcium release-activated calcium channel protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ORAI1