Orai1 Antibody (6D11A11) - BSA Free

Western Blot: Orai1 Antibody (6D11A11) [NBP1-75523] - analysis of ORAI1 in human spleen tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry-Paraffin: Orai1 Antibody (6D11A11) [NBP1-75523] - Human spleen tissue with ORAI1 antibody at 5 ug/mL.

Immunohistochemistry: Orai1 Antibody (6D11A11) - BSA Free [NBP1-75523] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: Orai1 Antibody (6D11A11) - BSA Free [NBP1-75523] - Immunofluorescence of Orai1 in Human Spleen tissue with Orai1 antibody at 20 ug/mL.

Immunohistochemistry: Orai1 Antibody (6D11A11) - BSA Free [NBP1-75523] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 5 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 6D11A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Orai1 Antibody (6D11A11) - BSA Free Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
• Specificity: ORAI1 antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ORAI1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Orai1 Antibody (6D11A11) - BSA Free

• CRACM1
• CRACM1ORAT1
• FLJ14466
• ORAI calcium release-activated calcium modulator 1
• Orai1
• ORAT1
• Protein orai-1
• TMEM142A
• TMEM142Acalcium release-activated calcium modulator 1
• Transmembrane protein 142Acalcium release-activated calcium channel protein 1

Background

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Orai1 Antibody (NBP1-75523)(2)

Publications using NBP1-75523

• Tandl D X-ray irradiation triggers via ROS production a canonical Ca2+-dependent immune response in T-lymphocytes Thesis 2021-01-01 (ICC/IF, Human)
• Tandl D, Sponagel T, Alansary D et al. X-ray irradiation triggers immune response in human T-lymphocytes via store-operated Ca2+ entry and NFAT activation The Journal of general physiology [PMID: 35416945] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: ORAI1