OLFM-L3 Antibody (740404) [Unconjugated]

Western blot shows lysates of mouse skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse OLFM-L3 Monoclonal Antibody (Catalog # MAB7324) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for OLFM-L3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 740404
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

OLFM-L3 Antibody (740404) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse OLFM-L3
  Gln22-Val406
  Accession # Q8BK62
• Specificity: Detects mouse OLFM-L3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: OLFML3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OLFM-L3 Antibody (740404) [Unconjugated]

• HNOEL-iso
• HNOEL-isoOLF44
• hOLF44
• OLF44
• olfactomedin-like 3
• olfactomedin-like protein 3
• OLFML3
• OLFM-L3

Background

OLFM-L3, also called OLF44, is an approximately 50 kDa secreted scaffolding glycoprotein of the Olfactomedin/Noelin family. It is predominantly expressed in the placenta and early embryo, but at low levels in the adult. In Xenopus it influences dorsoventral patterning during early development by restricting dorsal Chordin activity. Mature mouse OLFM-L3 shares 95% and 97% aa identity with human and mouse OLFM-L3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OLFML3
• Uniprot:
  • Q8BK62()