OGDH Antibody

Images

 
Western Blot: OGDH Antibody [NBP2-19622] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: OGDH Antibody [NBP2-19622] - OGDH antibody detects OGDH protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ...read more
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Mouse heart. OGDH stained by OGDH antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: OGDH Antibody [NBP2-19622] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: OGDH Antibody [NBP2-19622] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and blotted with OGDH antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) ...read more
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Cal27 xenograft, using OGDH antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Mouse muscle. OGDH antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Mouse muscle. OGDH stained by OGDH antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

OGDH Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human OGDH. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Predicted Species
Rat (93%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OGDH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (87%), Xenopus laevis (84%),

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for OGDH Antibody

  • 2-oxoglutarate dehydrogenase complex component E1
  • 2-oxoglutarate dehydrogenase, mitochondrial
  • AKGDH
  • alpha KGD
  • alpha-KGD
  • E1k
  • KGD1
  • OGDC
  • OGDC-E1
  • OGDH
  • OGDH2
  • oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
  • oxoglutarate decarboxylase
  • oxoglutarate dehydrogenase (succinyl-transferring)

Background

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol OGDH
Uniprot