OCT2 Overexpression Lysate (Native)

Images

 
Western Blot: OCT2 Overexpression Lysate (Native) [NBP2-10845] - OCT2 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Oct2.

Product Details

Summary
Applications WB

Order Details

OCT2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_002689. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens OCT2 Overexpression Lysate (Native), mRNA.
Gene
POU2F2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
49.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for OCT2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for OCT2 Overexpression Lysate (Native)

  • homeobox protein
  • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
  • OCT2Oct-2
  • Octamer-binding protein 2
  • Octamer-binding transcription factor 2
  • OTF-2
  • OTF2POU domain class 2, transcription factor 2
  • POU class 2 homeobox 2
  • POU domain, class 2, transcription factor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24677
Species: Hu, Ca, Eq, Pm
Applications: WB, IHC-P
NBP1-51684
Species: Hu
Applications: WB, ELISA, Flow
NBP2-45374
Species: Hu
Applications: WB, IHC
NB100-68210
Species: Hu, Mu
Applications: WB, IP
NBP1-89417
Species: Hu, Rt
Applications: WB, IHC, IHC-P
NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
NBP1-83976
Species: Hu
Applications: IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-27163
Species: Hu, Mu, Bv, Ca, Eq, Pm
Applications: WB, Flow
AF5486
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, CyTOF-ready, ICC
AF3487
Species: Hu
Applications: WB, IHC, ICC
NBP1-87909
Species: Hu
Applications: IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow
H00054474-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
H00009356-M05
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NBP2-10845
Applications: WB

Publications for OCT2 Lysate (NBP2-10845) (0)

There are no publications for OCT2 Lysate (NBP2-10845).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for OCT2 Lysate (NBP2-10845) (0)

There are no reviews for OCT2 Lysate (NBP2-10845). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for OCT2 Lysate (NBP2-10845) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional OCT2 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for OCT2 Lysate (NBP2-10845)

Discover related pathways, diseases and genes to OCT2 Lysate (NBP2-10845). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for OCT2 Lysate (NBP2-10845)

Discover more about diseases related to OCT2 Lysate (NBP2-10845).
 

Pathways for OCT2 Lysate (NBP2-10845)

View related products by pathway.

PTMs for OCT2 Lysate (NBP2-10845)

Learn more about PTMs related to OCT2 Lysate (NBP2-10845).
 

Research Areas for OCT2 Lysate (NBP2-10845)

Find related products by research area.

Blogs on OCT2

There are no specific blogs for OCT2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our OCT2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol POU2F2

Customers Who Bought This Also Bought

OCT4 Antibody
NB100-2379
OCT4 Antibody
NB100-2379
OCT4 Antibody
NB100-2379