Occludin Antibody

Western Blot: Occludin Antibody [H00004950-D01P] - Analysis of OCLN expression in mouse liver.

Western Blot: Occludin Antibody [H00004950-D01P] - Analysis of OCLN expression in human liver.

Western Blot: Occludin Antibody [H00004950-D01P] - Analysis of OCLN expression in transfected 293T cell line by OCLN polyclonal antibody.Lane 1: OCLN transfected lysate(59.10 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: Occludin Antibody [H00004950-D01P] - Analysis of protein-protein interactions between OCLN and CSNK1E. HeLa cells were stained with anti-OCLN rabbit purified polyclonal 1:1200 and anti-CSNK1E mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

Occludin Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: OCLN (NP_002529.1, 1 a.a. - 522 a.a.) full-length human protein. MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
• Marker: Tight Junctions Marker
• Specificity: OCLN - occludin,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OCLN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Occludin Antibody

• BLCPMG
• Occludin
• OCLN

Background

This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OCLN
• Entrez:
  • 4950(Human)
• Uniprot:
  • NP_002529.1(Human)