Occludin Antibody (690213) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 690213
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Occludin Antibody (690213) [Alexa Fluor® 647] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Occludin
  Accession # Q16625
• Specificity: Detects human Occludin in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Occludin Antibody (690213) [Alexa Fluor® 647]

• BLCPMG
• Occludin
• OCLN

Background

Occludin is an integral membrane protein with an apparent molecular mass of approximately 65 kDa.  It is localized exclusively at tight junctions (TJ) of select epithelial and endothelial cells.  The protein is 522 amino acids (aa) in length and contains a cytoplasmic N-terminus, four transmembrane domains, and a long COOH‑terminal cytoplasmic domain (domain E) that contains 255 aa.  Residues 1-66 make up the first cytoplasmic domain, and that is included in the MARVEL region consisting of aa 57-210.  The MARVEL region is a membrane-associating domain that spans aa 67-243.  Of note, residues 92-131 are glycine and tyrosine rich.  Residues 244-265 constitute the last transmembrane region, with aa 266-522 representing the long cytoplasmic domain termed domain E.  At the TJ, Occludin associates with membrane peripheral protein ZO-1 (220 kDa).  Human Occludin shares 90% aa sequence identity with mouse Occludin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.