OCAM/NCAM2 Antibody (125101)

• Reactivity: Mu
• Applications: WB
• Clone: 125101
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

OCAM/NCAM2 Antibody (125101) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse OCAM/NCAM2
  Leu20-Gly700 (predicted)
  Accession # O35136
• Specificity: Detects mouse OCAM/NCAM2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhMCAM, recombinant mouse (rm) NCAM-L1, or rmMAdCAM-1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: NCAM2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OCAM/NCAM2 Antibody (125101)

• NCAM2
• N-CAM-2
• NCAM-2
• NCAM21MGC51008
• neural cell adhesion molecule 2 precursor10
• neural cell adhesion molecule 2
• OCAM
• Rb-8
• RNCAM

Background

OCAM, also known as neural cell adhesion molecule 2 (NCAM2), belongs to the immunoglobulin superfamily. It exists either as a type I membrane protein or is attached to the membrane via a GPI-anchor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OCAM/NCAM2 Antibody (MAB778)(3)

Publications using MAB778

• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020-10-14;11(1):5171. 2020-10-14 [PMID: 33057002] (IHC, Mouse)
• Iryna Leshchyns’ka, Heng Tai Liew, Claire Shepherd, Glenda M. Halliday, Claire H. Stevens, Yazi D. Ke et al. A beta -dependent reduction of NCAM2-mediated synaptic adhesion contributes to synapse loss in Alzheimer’s disease Nature Communications 2015-11-27 [PMID: 26611261]
• Deleyrolle L, Sabourin J, Rothhut B, Fujita H, Guichet P, Teigell M, Ripoll C, Chauvet N, Perrin F, Mamaeva D, Noda T, Mori K, Yoshihara Y, Hugnot J OCAM Regulates Embryonic Spinal Cord Stem Cell Proliferation by Modulating ErbB2 Receptor. PLoS ONE, 2015-04-13;10(4):e0122337. 2015-04-13 [PMID: 25875008] (IHC, Mouse)

Bioinformatics

• Gene Symbol: NCAM2
• Uniprot:
  • O35136()