O-GlcNAc Transferase p110 subunit Antibody (JB44-39)

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of OGT on Hela (1) and SiHa (2) cell lysate using anti-OGT antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Staining OGT in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of paraffin-embedded rat epididymis tissue using anti-OGT antibody. Counter stained with hematoxylin.

Flow Cytometry: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of Hela cells with OGT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Staining OGT in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Staining OGT in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of paraffin-embedded human liver tissue using anti-OGT antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of paraffin-embedded human kidney tissue using anti-OGT antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of paraffin-embedded mouse testis tissue using anti-OGT antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JB44-39
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

O-GlcNAc Transferase p110 subunit Antibody (JB44-39) Summary

• Description: Novus Biologicals Rabbit O-GlcNAc Transferase p110 subunit Antibody (JB44-39) (NBP2-75601) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human O-GlcNAc Transferase p110 subunit aa 997-1,046 / 1,046. (SwissProt: O15294 Human; SwissProt: Q8CGY8 Mouse; SwissProt: P56558 Rat)
• Localization: Nucleus. Cytoplasm. Membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: OGT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 117 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for O-GlcNAc Transferase p110 subunit Antibody (JB44-39)

• EC 2.4.1
• EC 2.4.1.255
• FLJ23071
• HRNT1
• MGC22921
• O-GlcNAc transferase p110 subunit
• O-GlcNAc transferase subunit p110
• OGlcNAc Transferase
• O-GlcNAc Transferase
• O-GLCNAC
• OGT
• O-linked N-acetylglucosamine (GlcNAc) transferase(UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• O-linked N-acetylglucosamine transferase 110 kDa subunit
• UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDasubunit
• uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase

Background

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OGT