O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:500. Blue: Fluoroshield with DAPI .

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc Transferase p110 subunit antibody [HL1641] (NBP3-25586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Dr
• Applications: WB, ICC/IF, IHC
• Clone: HL1641
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free (NBP3-25586) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human O-GlcNAc Transferase p110 subunit. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: OGT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

• EC 2.4.1
• EC 2.4.1.255
• FLJ23071
• HRNT1
• MGC22921
• O-GlcNAc transferase p110 subunit
• O-GlcNAc transferase subunit p110
• OGlcNAc Transferase
• O-GlcNAc Transferase
• O-GLCNAC
• OGT
• O-linked N-acetylglucosamine (GlcNAc) transferase(UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• O-linked N-acetylglucosamine transferase 110 kDa subunit
• UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDasubunit
• uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase

Background

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OGT