Nuf2 Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 488

Nuf2 Antibody [Alexa Fluor™ Plus 488] Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 414 to 464 of human Nuclear Fallout 2 using the numbering given in entry NP_663735.2 (GeneID 83540).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Nuf2 Antibody [Alexa Fluor™ Plus 488]

• cancer/testis antigen 106
• CDCA1hsNuf2
• cell division cycle associated 1
• Cell division cycle-associated protein 1
• CT106
• hNuf2
• kinetochore protein Nuf2
• NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
• NUF2RhNuf2R

Background

Nuf2, also known as Kinetochore protein Nuf2, is a 54 kDa 464 amino acid protein, and it is involved in chromosome segregation, specifically as it relates to spindle checkpoint activity with the kinetochore-associated NDC80 complex. This protein is necessary for kinetochore stability and organization of microtubule binding sites on the outer plate, forming interactions with NDC80/HEC-1-CDCA1, SPBC24-SPBC25, Bub1, EXOC1, as well as AURKB/Aurora-B and CENPE in the mitotic prometaphase, DNA replication, and Cell Cycle pathways. Current research is being performed on this proteins involvement in gastric, lung, and breast cancers, in addition to essential hypertension, schizophrenia, and duodenogastric reflux.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUF2