Recombinant Human NUDT21 His Protein

SDS-Page: Recombinant Human NUDT21 Protein [NBP1-51010] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human NUDT21 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-227 of Human NUDT21
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NUDT21
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 28.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 2 mM DTT, 20% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human NUDT21 His Protein

• CFIm25
• CFIM25CPSF 25 kDa subunit
• cleavage and polyadenylation specific factor 5, 25 kD subunit
• cleavage and polyadenylation specific factor 5, 25 kDa
• Cleavage and polyadenylation specificity factor 25 kDa subunit
• cleavage and polyadenylation specificity factor subunit 5
• CPSF25
• CPSF5DKFZp686H1588
• Nucleoside diphosphate-linked moiety X motif 21
• nudix (nucleoside diphosphate linked moiety X)-type motif 21
• Nudix motif 21
• pre-mRNA cleavage factor Im (25kD)
• Pre-mRNA cleavage factor Im 25 kDa subunit
• pre-mRNA cleavage factor Im, 25kD subunit

Background

NUDT21, also known as CPSF5 or CFIm25, is a member of the Nudix hydrolase family of pyrophosphatases. This protein localizes to the paraspeckles and forms a heterodimer with CPSF6 or CPSF7 to comprise the CFIm (mammalian cleavage factor I) complex. NUDT21 is the smaller subunit of the complex and is present in all heterodimer combinations. NUDT21 plays an important role in pre-mRNA 3' cleavage and polyadenylation processing. Recombinant human NUDT21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NUDT21