NUDT2 Overexpression Lysate

Western Blot: NUDT2 Overexpression Lysate (Adult Normal) [NBL1-13860] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NUDT2.

• Reactivity: Hu
• Applications: WB

NUDT2 Overexpression Lysate Summary

• Description:

  NUDT2 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC217288
  
  Accession#: NM_001161
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: NUDT2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 16.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for NUDT2 Overexpression Lysate

• Ap4A hydrolase 1
• Ap4A hydrolase
• Ap4Aase
• APAH1Diadenosine 5'-5'''-P1
• bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
• bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
• diadenosine 5'-5''-P1
• Diadenosine tetraphosphatase
• EC 3.6.1.17
• MGC10404
• Nucleoside diphosphate-linked moiety X motif 2
• nudix (nucleoside diphosphate linked moiety X)-type motif 2
• Nudix motif 2
• P4-tetraphosphate asymmetrical hydrolase
• P4-tetraphosphate pyrophosphohydrolase

Background

NUDT2 encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and three transcript variants, all encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: NUDT2