NUDT16 Antibody

Immunocytochemistry/Immunofluorescence: NUDT16 Antibody [NBP2-37976] - Staining of human cell line MCF7 shows positivity in nucleus and nucleoli.

Immunohistochemistry-Paraffin: NUDT16 Antibody [NBP2-37976] - Staining of human appendix shows moderate nucleolar positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NUDT16 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: VERPDYRSSHAGSRPRVVAHFYAKSL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUDT16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NUDT16 Protein (NBP2-37976PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NUDT16 Antibody

• EC 3.6.1
• EC 3.6.1.-
• FLJ31265
• FLJ34034
• FLJ36248
• Nucleoside diphosphate-linked moiety X motif 16
• nudix (nucleoside diphosphate linked moiety X)-type motif 16
• Nudix motif 16
• U8 snoRNA-binding protein H29K
• U8 snoRNA-decapping enzyme

Background

RNA-decapping enzyme that binds specifically to U8 snoRNA. Part of the U8 snoRNP complex that is required forthe accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and m227G caps from U8snoRNA. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species (invitro)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUDT16
• Uniprot:
  • Q96DE0(Human)