Nudel Overexpression Lysate

Images

 
Western Blot: Nudel Overexpression Lysate (Adult Normal) [NBL1-13523] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nudel.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Nudel Overexpression Lysate Summary

Description

Nudel Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC205974

Accession#: NM_030808

Protein Tag: C-MYC/DDK
Gene
NDEL1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
38.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Nudel Overexpression Lysate

  • DKFZp451M0318
  • EOPA
  • MITAP1mitosin-associated protein MITAP1
  • Mitosin-associated protein 1
  • nuclear distribution protein nudE-like 1
  • nudE nuclear distribution gene E homolog (A. nidulans)-like 1
  • nudE nuclear distribution gene E homolog like 1 (A. nidulans)
  • nudE nuclear distribution gene E homolog like 1
  • NUDELendooligopeptidase A
  • NUDE-like protein
  • Protein Nudel

Background

This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an alternate exon, compared to variant 1. The resulting protein (isoform B) is longer and has a distinct C-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Nudel Lysate (NBL1-13523) (0)

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Bioinformatics

Gene Symbol NDEL1