Nudel Overexpression Lysate

Western Blot: Nudel Overexpression Lysate (Adult Normal) [NBL1-13523] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nudel.

• Reactivity: Hu
• Applications: WB

Nudel Overexpression Lysate Summary

• Description:

  Nudel Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC205974
  
  Accession#: NM_030808
  
  Protein Tag: C-MYC/DDK
• Gene: NDEL1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 38.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Nudel Overexpression Lysate

• DKFZp451M0318
• EOPA
• MITAP1mitosin-associated protein MITAP1
• Mitosin-associated protein 1
• nuclear distribution protein nudE-like 1
• nudE nuclear distribution gene E homolog (A. nidulans)-like 1
• nudE nuclear distribution gene E homolog like 1 (A. nidulans)
• nudE nuclear distribution gene E homolog like 1
• NUDELendooligopeptidase A
• NUDE-like protein
• Protein Nudel

Background

This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an alternate exon, compared to variant 1. The resulting protein (isoform B) is longer and has a distinct C-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: NDEL1