NUDC Antibody - Azide and BSA Free

Western Blot: NUDC Antibody [NBP2-96997] - Anti-NUDC rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: NIH3T3 Whole Cell Lysate. Lane D: Raw264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 46 kDa

Immunohistochemistry-Paraffin: NUDC Antibody [NBP2-96997] - Staining of human NUDC in human kidney with rabbit polyclonal antibody (1:5000).

Immunohistochemistry-Paraffin: NUDC Antibody [NBP2-96997] - Staining of human NUDC in human brain with rabbit polyclonal antibody (1:5000).

Immunoprecipitation: NUDC Antibody [NBP2-96997] - NUDC-His(C) was immunoprecipitated using: Lane A: 0.5 mg Raw264.7 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg Hela Whole Cell Lysate4 ul anti-NUDC-His(C) rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NUDC-His(C) rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 42 kDa

Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP2-96997] - Immunofluorescence staining of NUDC in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-96997 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NUDC Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human NUDC (Accession#: NP_006591.1; Met1-Asn331)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUDC
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunohistochemistry-Paraffin 1:2500-1:10000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for NUDC Antibody - Azide and BSA Free

• HNUDC
• MNUDC
• NPD011
• nuclear distribution gene C (A.nidulans) homolog
• nuclear distribution gene C homolog (A. nidulans)
• Nuclear distribution protein C homolog
• nuclear migration protein nudC
• NudC

Background

NudC was first identified as a regulator of nuclear movement in the asexual reproductive cycle of the filamentous fungus Aspergillus nidulans. Human NUDC is a nuclear movement protein that associates with dynein (see DYNC1H1; MIM 600112) (Aumais et al., 2003 [PubMed 12679384]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUDC