![Western Blot: Nucleoplasmin-2 Antibody (5E9) [H00010361-M03] - Analysis of NPM2 expression in transfected 293T cell line by NPM2 monoclonal antibody (M03), clone 5E9. Lane 1: NPM2 transfected lysate (Predicted MW: 24.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/Nucleoplasmin-2 Antibody (5E9)-Western Blot-H00010361-M03-img0003.jpg "Western Blot: Nucleoplasmin-2 Antibody (5E9) [H00010361-M03] - Analysis of NPM2 expression in transfected 293T cell line by NPM2 monoclonal antibody (M03), clone 5E9. Lane 1: NPM2 transfected lysate (Predicted MW: 24.2 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 5E9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | NPM2 (NP_877724.1, 1 a.a. ~ 214 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Specificity | This product is specific for Human NPM2 monoclonal antibody (M03), clone 5E9 [Gene ID: 10361]. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NPM2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Mouse monoclonal antibody raised against a full-length recombinant NPM2. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Diseases for Nucleoplasmin-2 Antibody (H00010361-M03)Discover more about diseases related to Nucleoplasmin-2 Antibody (H00010361-M03).
| Pathways for Nucleoplasmin-2 Antibody (H00010361-M03)View related products by pathway.
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PTMs for Nucleoplasmin-2 Antibody (H00010361-M03)Learn more about PTMs related to Nucleoplasmin-2 Antibody (H00010361-M03).
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| Gene Symbol | NPM2 |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Nucleoplasmin-2 Antibody (5E9) [H00010361-M03] - Analysis of monoclonal antibody to NPM2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize2/Nucleoplasmin-2-Antibody-(5E9)-Immunocytochemistry-Immunofluorescence-H00010361-M03-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nucleoplasmin-2 Antibody (5E9) [H00010361-M03] - Analysis of monoclonal antibody to NPM2 on HeLa cell. Antibody concentration 10 ug/ml")
![ELISA: Nucleoplasmin-2 Antibody (5E9) [H00010361-M03] - Detection limit for recombinant GST tagged NPM2 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/Nucleoplasmin-2-Antibody-(5E9)-ELISA-H00010361-M03-img0004.jpg "ELISA: Nucleoplasmin-2 Antibody (5E9) [H00010361-M03] - Detection limit for recombinant GST tagged NPM2 is 3 ng/ml as a capture antibody.")
![Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.](http://images.novusbio.com/fullsize2/Nucleophosmin-Antibody-Western-Blot-NB110-61646-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).](http://images.novusbio.com/fullsize2/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.](http://images.novusbio.com/fullsize2/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0010.jpg)
![Western Blot: Nucleoplasmin-3 Antibody [NBP1-90999] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/Nucleoplasmin-3-Antibody-Western-Blot-NBP1-90999-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleoplasmin-3 Antibody [NBP1-90999] - Staining of human vulva/anal skin shows strong nucleolar and cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize2/Nucleoplasmin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-90999-img0002.jpg)
![Western Blot: ZAR1 Overexpression Lysate (Adult Normal) [NBL1-17958] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZAR1.](http://images.novusbio.com/fullsize/ZAR1 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-17958-img0002.jpg)
![Western Blot: NLRP5 Antibody [NBP1-76289] - Mouse brain tissue lysate with NLRP5 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize2/NLRP5-Antibody-Western-Blot-NBP1-76289-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP5 Antibody [NBP1-76289] - Immunofluorescence of NALP5 in human brain tissue with NALP5 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize2/NLRP5-Antibody-Immunofluorescence-NBP1-76289-img0004.jpg)
![Immunohistochemistry: NLRP5 Antibody [NBP1-76289] - Immunohistochemistry of NALP5 in human brain tissue with NALP5 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize2/NLRP5-Antibody-Immunohistochemistry-NBP1-76289-img0003.jpg)
![Immunohistochemistry-Paraffin: ZP4 Antibody [NBP1-86823] - Staining of human ovary shows cytoplasmic positivity in oocytes.](http://images.novusbio.com/fullsize2/ZP4 Antibody-Immunohistochemistry-Paraffin-NBP1-86823-img0006.jpg)
![Western Blot: NASP Antibody [NBP2-33928] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-NASP antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize2/NASP-Antibody-Western-Blot-NBP2-33928-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: NASP Antibody [NBP2-33928] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/NASP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33928-img0014.jpg)
![Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928] - Staining in human testis and pancreas tissues using anti-NASP antibody. Corresponding NASP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/NASP-Antibody-Immunohistochemistry-Paraffin-NBP2-33928-img0013.jpg)
![Western Blot: Geminin Antibody (1A8) [H00051053-M01] - Analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody (M01), clone 1A8.Lane 1: GMNN transfected lysate(23.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/Geminin Antibody (1A8)-Western Blot-H00051053-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/Geminin-Antibody-(1A8)-Immunocytochemistry-Immunofluorescence-H00051053-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize2/Geminin-Antibody-(1A8)-Immunohistochemistry-Paraffin-H00051053-M01-img0010.jpg)
![Western Blot: NOBOX Antibody (4C5) [NBP2-46193] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOBOX.](http://images.novusbio.com/fullsize2/NOBOX Antibody (4C5)-Western Blot-NBP2-46193-img0006.jpg)
![Immunohistochemistry: NOBOX Antibody (4C5) [NBP2-46193] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize2/NOBOX-Antibody-(4C5)-Immunohistochemistry-NBP2-46193-img0005.jpg)
![Immunohistochemistry: NOBOX Antibody (4C5) [NBP2-46193] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize2/NOBOX-Antibody-(4C5)-Immunohistochemistry-NBP2-46193-img0001.jpg)
![Immunohistochemistry-Paraffin: ZP3 Antibody [NBP2-30830] - Staining of human ovary shows strong cytoplasmic positivity in follicle cells.](http://images.novusbio.com/fullsize2/ZP3-Antibody-Immunohistochemistry-Paraffin-NBP2-30830-img0003.jpg)
![Western Blot: ZP2 Antibody [NBP1-86103] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/ZP2-Antibody-Western-Blot-NBP1-86103-img0005.jpg)
![Immunohistochemistry-Paraffin: ZP2 Antibody [NBP1-86103] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/ZP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86103-img0002.jpg)
![Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/AICDA Antibody-Western Blot-NBP1-30955-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/AICDA Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30955-img0002.jpg)
![Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded mouse lymph node. AICDA antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize2/AICDA-Antibody-Immunohistochemistry-Paraffin-NBP1-30955-img0005.jpg)
![Western Blot: TACC3 Antibody (6F1) [NBP2-02619] Analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3 Antibody (6F1)-Western Blot-NBP2-02619-img0010.jpg)
![Immunohistochemistry-Paraffin: TACC3 Antibody (6F1) [NBP2-02619] - Staining of paraffin-embedded Human prostate tissue using anti-TACC3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TACC3 Antibody (6F1)-Immunohistochemistry-Paraffin-NBP2-02619-img0009.jpg)
![Flow Cytometry: TACC3 Antibody (6F1) [NBP2-02619] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TACC3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TACC3 Antibody (6F1)-Flow Cytometry-NBP2-02619-img0001.jpg)
![Western Blot: Lysozyme Antibody [NBP2-61118] - Total protein from human cell lines THP-1 and HepG2, human stomach and kidney as well as mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Lysozyme in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Western-Blot-NBP2-61118-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Lysozyme Antibody [NBP2-61118] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Lysozyme at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61118-img0002.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-61118] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) tissue section of human kidney with Lysozyme antibody at 1:100 dilution. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This Lysozyme antibody generated an expected cytoplasmic staining in all the cells with strongest signal in tubular epithelial cells. Some tubules showed more of a punctate staining pattern (vesicular Lysozyme) while all of the tubules depicted a diffused signal (secreted Lysozyme). A subset of cells, especially those from glomeruli, showed nuclear positivity also.](http://images.novusbio.com/fullsize2/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-61118-img0001.jpg)
![Western Blot: SCD Antibody [NBP1-26393] - Normal human liver.](http://images.novusbio.com/fullsize2/SCD Antibody-Western Blot-NBP1-26393-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SCD Antibody [NBP1-26393] - SCD staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize2/SCD Antibody-Immunocytochemistry-Immunofluorescence-NBP1-26393-img0004.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 647 conjugate of NBP1-43319. Mouse IgG1 isotype control was used as a negative control. Image from verified customer review.](http://images.novusbio.com/images2/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-(P3.6.2.8.1)-Flow-Cytometry-NBP1-43319-img0001.jpg "Flow Cytometry: Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 647 conjugate of NBP1-43319. Mouse IgG1 isotype control was used as a negative control. Image from verified customer review.")