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Nucleoplasmin-3 Antibody

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Western Blot: Nucleoplasmin-3 Antibody [NBP1-90999] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: Nucleoplasmin-3 Antibody [NBP1-90999] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate ...read more
Immunohistochemistry-Paraffin: Nucleoplasmin-3 Antibody [NBP1-90999] - Staining of human vulva/anal skin shows strong nucleolar and cytoplasmic positivity in squamous epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Nucleoplasmin-3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ARNHDHQEIAVPVANLKLSCQPMLSLDDFQLQPPVTFRLKSGSGPVRITGRHQIVTMSNDVSE
Specificity
Specificity of human Nucleoplasmin-3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPM3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Positive Control
Nucleoplasmin-3 Lysate (NBP2-66010)
Control Peptide
Nucleoplasmin-3 Protein (NBP1-90999PEP)
Publications
Read Publication using
NBP1-90999 in the following applications:

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (87%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Nucleoplasmin-3 Antibody

  • nucleophosmin/nucleoplasmin 3
  • nucleophosmin/nucleoplasmin family, member 3
  • nucleoplasmin-3
  • PORMIN
  • TMEM123

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Nucleoplasmin-2 Antibody [NBP2-13673] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli.
Immunohistochemistry-Paraffin: Nucleoplasmin-2 Antibody [NBP2-13673] - Staining in human parathyroid gland and liver tissues using anti-NPM2 antibody. Corresponding NPM2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Nucleoplasmin-2 Antibody [NBP2-13673] - Staining of human pancreas shows strong nuclear positivity in islet cells.
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Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
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Immunohistochemistry: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of paraffin-embedded colon cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Immunofluorescence: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Western Blot: MMTV Antibody [NBP2-44179] - lane 1: cell lysate negative control. Lane 2: cell lysate spiked with purified virus. Load: 10 Ug per lane.
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Nucleoplasmin-3
Antibody
NBP1-90999
Nucleoplasmin-3 Antibody
Species: Hu
Applications: WB, IHC, IHC-P

Publications for Nucleoplasmin-3 Antibody (NBP1-90999)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Positive Control Lysate(s)

Nucleoplasmin-3 Knockout 293T Cell Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol NPM3