Nuclear Factor Erythroid Derived 2 Antibody

Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NF-E2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-31540] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-E2 protein stained by NF-E2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

• Reactivity: Hu, Rt, Po, Bv, RM
• Applications: WB, ICC/IF, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Nuclear Factor Erythroid Derived 2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Nuclear Factor Erythroid Derived 2. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (90%), Porcine (91%), Bovine (90%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFE2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
• Application Notes: ChIP-seq, IP Assay dependent
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Nuclear Factor Erythroid Derived 2 Antibody

• Leucine zipper protein NF-E2
• NF-E2
• nuclear factor (erythroid-derived 2), 45kD
• nuclear factor (erythroid-derived 2), 45kDa
• Nuclear factor, erythroid-derived 2 45 kDa subunit
• p45 NF-E2
• p45
• transcription factor NF-E2 45 kDa subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NFE2