NTF2 Antibody (4F5)

Product Discontinued

NTF2 Antibody (4F5) Summary

• Immunogen: Recombinant full length protein (Human).
• Epitope: The epitope recognized by the antibody lies within the extreme C-terminus of NTF2.
• Localization: Cytoplasmic
• Specificity: NTF2 (4F5)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NUTF2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1-2 ug/ml
• Application Notes: WB:Detects a band of approximately 14 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Cross-reacts with Human, Mouse, Rat, Xenopus laevis, Chicken and Monkey. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for NTF2 Antibody (4F5)

• NTF-2
• NTF2PP15Placental protein 15
• nuclear transport factor 2

Background

The protein encoded by the NTF2 gene is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62. This encoded protein acts at a relative late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It is thought to be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUTF2
• Entrez:
  • 10204(Human)
  • 68051(Mouse)
  • 291981(Rat)