NTAL Antibody (NAP-03)

Product Discontinued

NTAL Antibody (NAP-03) Summary

• Immunogen: Peptide corresponding to aa 196-212 of human NTAL coupled to KLH.
• Specificity: The antibody NAP-03 recognizes the defined epitope (aa 196-212) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1-2 ug/ml
• Application Notes: Predicted molecular weight 30 kDa. Western Blot: incubation 60 min Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Non-Reducing conditions. 12% separating SDS-PAGE gel.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for NTAL Antibody (NAP-03)

• LAB
• LABWilliams-Beuren syndrome chromosomal region 15 protein
• Linker for activation of B-cells
• linker for activation of T cells family, member 2
• linker for activation of T-cells family member 2
• Membrane-associated adapter molecule
• Non-T-cell activation linker
• NTAL
• NTALWilliams-Beuren syndrome chromosomal region 5 protein
• WBS15
• WBSCR15
• WBSCR5
• WBSCR5HSPC046
• Williams-Beuren syndrome chromosome region 5
• WSCR5

Background

The adaptor protein NTAL (Non-T cell Activation Linker; human 30 kDa, mouse 25 kDa), alternatively termed as linker for activation of B cells (LAB), is expressed in plasma membrane lipid rafts of B cells, mast cells, monocytes and NK cells. The engagement and activation of antigen receptors on B and T lymphocytes initiates signal transducing pathways. These pathways are vital for the successful initiation of an adaptive immune response and it is following the cross-linking of the B cell receptor (BCR) or high-affinity FE receptors of myeloid cells, that the tyrosine phosphorylation of NTAL occurs, enabling the formation of complexes between NTAL and further signaling molecules Grb2, Gab1, Sos1 and c-Cbl. Antigen-initiated activation events include tyrosine phosphorylation of two transmembrane adaptor proteins, LAT and NTAL; however NTAL is functionally and topographically different from LAT. Recent studies have indicated, that the evidence for functional homology between LAT and NTAL/LAB, and the possibility that the differences between them might lead to specific clinical therapeutics to manipulate immune cell responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAT2