NSMAF Overexpression Lysate

Images

 
Western Blot: NSMAF Overexpression Lysate (Adult Normal) [NBL1-13811] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for NSMAF.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NSMAF Overexpression Lysate Summary

Description
Transient overexpression lysate of neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC207925 and based on accession number NM_003580. The protein contains a C-MYC/DDK Tag.
Gene
NSMAF

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
104.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NSMAF Overexpression Lysate

  • Factor associated with neutral sphingomyelinase activation
  • FANFactor associated with N-SMase activation
  • neutral sphingomyelinase (N-SMase) activation associated factor
  • protein FAN

Background

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants. Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Other Available Formats

NBL1-13811

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Bioinformatics

Gene Symbol NSMAF