NSF Antibody (3184A) [Unconjugated]

Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/ml of Rabbit Anti-Human NSF Monoclonal Antibody (Catalog # MAB11766) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for NSF at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 3184A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NSF Antibody (3184A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic Peptide
  Accession # P46459
• Specificity: Detects a synthetic peptide specific for human NSF around amino acid 520 in direct ELISA
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NSF Antibody (3184A) [Unconjugated]

• DEE96
• EC 3.6.4.6
• NEM-sensitive fusion protein
• N-ethylmaleimide-sensitive factor
• N-ethylmaleimide-sensitive factor-like protein
• N-ethylmaleimide-sensitive fusion protein
• NSF
• SEC18
• SKD2
• vesicle-fusing ATPase
• Vesicular-fusion protein NSF

Background

N-ethylmaleimide-sensitive factor (NSF) is an ATPase of the AAA+ protein family, with a molecular weight of approximately 83 kDa. NSF is a key component of membrane trafficking pathways and plays a central role in the disassembly of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, thereby facilitating vesicle fusion and neurotransmitter release. It is ubiquitously expressed across cell types and is essential for intracellular trafficking processes such as endocytosis, exocytosis, and autophagosome formation. NSF's functionality extends to maintaining the proper organization and dynamics of intracellular membranes, making it crucial for cellular homeostasis. Dysregulation of NSF activity has been implicated in various pathological conditions, including neurodegenerative disorders, where defects in vesicle fusion processes are linked to cognitive impairment. Additionally, NSF influences the recycling of neurotransmitter receptors and may play a role in synaptic plasticity, highlighting its importance in neuronal function and its potential as a therapeutic target for neurological diseases.

1. Hanson PI, Whiteheart SW. AAA+ proteins: have engine, will work. Nat Rev Mol Cell Biol. 2005 Jul;6(7):519-29. doi: 10.1038/nrm1684. PMID: 16072036.
2. Yang J, Kong L, Zou L, Liu Y. The role of synaptic protein NSF in the development and progression of neurological diseases. Front Neurosci. 2024 Oct 21;18:1395294. doi: 10.3389/fnins.2024.1395294. PMID: 39498393; PMCID: PMC11532144.
3. Zhao M, Brunger AT. Recent Advances in Deciphering the Structure and Molecular Mechanism of the AAA+ ATPase N-Ethylmaleimide-Sensitive Factor (NSF). J Mol Biol. 2016 May 8;428(9 Pt B):1912-26. doi: 10.1016/j.jmb.2015.10.026. Epub 2015 Nov 3. PMID: 26546278; PMCID: PMC4854814.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P46459(Human)