NSD3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NSD3 Antibody [NBP1-33013] - NSD3 antibody [C1C2], Internal detects NSD3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NSD3 protein stained by NSD3 antibody [C1C2], Internal (NBP1-33013) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 um.

Western Blot: NSD3 Antibody [NBP1-33013] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NSD3 antibody [C1C2], Internal (NBP1-33013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: NSD3 Antibody [NBP1-33013] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NSD3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NSD3 Antibody - BSA Free (NBP1-33013) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NSD3. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NSD3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 162 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NSD3 Antibody - BSA Free

• EC 2.1.1.43
• FLJ20353
• histone-lysine N-methyltransferase NSD3
• MGC126766
• MGC142029
• NSD3DKFZp667H044
• Nuclear SET domain-containing protein 3
• pp14328
• Protein whistle
• WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
• WHSC1-like protein 1
• Wolf-Hirschhorn syndrome candidate 1-like 1
• Wolf-Hirschhorn syndrome candidate 1-like protein 1

Background

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NSD3
• Entrez:
  • 54904(Human)
• Uniprot:
  • Q9BZ95(Human)