Nrf2 [p Ser40] Antibody (EP1809Y)

Product Discontinued

Nrf2 [p Ser40] Antibody (EP1809Y) Summary

• Immunogen: A phospho specific peptide corresponding to residues surrounding Serine 40 of human Nrf2 was used as an immunogen.
• Modification: p Ser40
• Specificity: This antibody only detects Nrf2 phosphorylated at Serine 40.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFE2L2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1/100
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:40
  • Western Blot 1:1000-10000
• Application Notes: In Western blot this antibody detects a band at approximately 100 kDa.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25778901)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Nrf2 [p Ser40] Antibody (EP1809Y)

• HEBP1
• NFE2L2
• NF-E2-related factor 2
• NFE2-related factor 2
• NRF2 erythroid derived 2, like 2
• Nrf2
• nuclear factor (erythroid-derived 2)-like 2
• nuclear factor erythroid 2-related factor 2
• nuclear factor erythroid-derived 2-like 2

Background

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress (1). Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin (2). Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nrf2 Antibody (NB100-80012)(7)

Publications using NB100-80012

• Yang JH, Na CS, Cho SS et al. Hepatoprotective Effect of Neoagarooligosaccharide via Activation of Nrf2 and Enhanced Antioxidant Efficacy Biol. Pharm. Bull. 2020-01-31 [PMID: 32009027] (WB, Human)
• Stanley A, Heo SJ, Mauck RL et al. Cudrania Tricuspidata Extract and Its Major Constituents Inhibit Oxidative Stress-Induced Liver Injury J Med Food 2019-05-02 [PMID: 31045470]
• Seo K, Ki SH, Shin SM. Sestrin2-AMPK activation protects mitochondrial function against glucose deprivation-induced cytotoxicity. Cell. Signal. 2015-03-14 [PMID: 25778901] (WB, Human, Mouse)
• Lee KM, Kang K, Lee SB, Nho CW. Nuclear factor-E2 (Nrf2) is regulated through the differential activation of ERK1/2 and PKC alpha/betaII by Gymnasterkoreayne B Cancer Lett 2012-12-05 [PMID: 23219897] (WB, ICC/IF, Human)
• Kay HY, Won Yang J, Kim TH et al. Ajoene, a stable garlic by-product, has an antioxidant effect through Nrf2-mediated glutamate-cysteine ligase induction in HepG2 cells and primary hepatocytes. J Nutr 2010-07-01 [PMID: 20463144] (WB, Human)
• Lee AC, Murray M et al. Up-regulation of human CYP2J2 in HepG2 cells by butylated hydroxyanisole is mediated by c-Jun and Nrf2. Mol Pharmacol 2010-06-01 [PMID: 20194533]
• Wojewoda M, Duszy?ski J, Szczepanowska J et al. Antioxidant defence systems and generation of reactive oxygen species in osteosarcoma cells with defective mitochondria: effect of selenium. Biochim Biophys Acta 2010-06-01 [PMID: 20138159]

Bioinformatics

• Gene Symbol: NFE2L2
• Entrez:
  • 4780(Human)
• Uniprot:
  • Q16236()