NrCAM Antibody (S364-51) - BSA Free

Western Blot: NrCAM Antibody (S364-51) [NBP2-42191] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (NBP2-42191). Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (NBP2-42191) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.

Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (NBP2-42191). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (NBP2-42191) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) NrCAM Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.

Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (NBP2-42191). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (NBP2-42191) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NrCAM Antibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: S364-51
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NrCAM Antibody (S364-51) - BSA Free Summary

• Description: Novus Biologicals Mouse NrCAM Antibody (S364-51) - BSA Free (NBP2-42191) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.
• Localization: Cell Membrane
• Specificity: Approx 50% identity with NeurofascinDetects approx 160kDa.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NRCAM
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of NrCAM Antibody was sufficient for detection of NrCAM in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NrCAM Antibody (S364-51) - BSA Free

• Bravo
• hBravo
• KIAA0343Neuronal surface protein Bravo
• MGC138845
• MGC138846
• neuronal cell adhesion molecule
• NgCAM-related cell adhesion molecule
• ng-CAM-related
• NrCAM
• nr-CAM

Background

NRCAM - neuronal cell adhesion molecule

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NRCAM
• Uniprot:
  • Q810U4()