NRAS Antibody

Images

 
Western Blot: NRAS Antibody [NB100-94887] - (0.01ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NRAS Antibody [NB100-94887] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: NRAS Antibody [NB100-94887] - (0.1ug/ml) staining of Rat Intestines lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NRAS Antibody Summary

Immunogen
Peptide with sequence C-RMKKLNSSDDGTQ corresponding to internal region according to NP_002515.1.
Epitope
C-RMKKLNSSDDGTQ
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NRAS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: Approx. 21kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2kDa according to NP_002515.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 for this particular batch.
Control
NRAS Lysate (NBL1-13785)
NRAS Lysate (NBP2-65959)
NRAS Lysate (NBP2-66265)
Publications
Read Publication using NB100-94887.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NRAS Antibody

  • ALPS4
  • GTPase NRas
  • HRAS1
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • N-ras protein part 4
  • N-ras
  • NRAS1
  • NS6
  • Transforming protein N-Ras
  • v-ras neuroblastoma RAS viral oncogene homolog

Background

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NRAS Antibody (NB100-94887)(1)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NRAS
Entrez
Uniprot