NPM1 Antibody (NPM1/3287) - Azide and BSA Free

Images

 
Western Blot: NPM1 Antibody (NPM1/3287) - Azide and BSA Free [NBP2-79876] - Western Blot Analysis of K562 cell lysate using NPM1 Antibody (NPM1/3287).
Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/3287) - Azide and BSA Free [NBP2-79876] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with NPM1 Antibody (NPM1/3287).
Protein Array: NPM1 Antibody (NPM1/3287) - Azide and BSA Free [NBP2-79876] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NPM1 Antibody (NPM1/3287) Z- and S- Score: The Z-score ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, PA
Clone
NPM1/3287
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

NPM1 Antibody (NPM1/3287) - Azide and BSA Free Summary

Immunogen
Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)
Localization
Nuclear, Cytoplasmic
Specificity
This antibody recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM1). It is predominantly localized in the nucleus of cells in most tissues. NPM1 is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NPM1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 2-4 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for NPM1 Antibody (NPM1/3287) - Azide and BSA Free

  • B23
  • MGC104254
  • NPM1
  • NPMnucleophosmin/nucleoplasmin family, member 1
  • Nucleolar phosphoprotein B23
  • Nucleolar protein NO38
  • nucleophosmin (nucleolar phosphoprotein B23, numatrin)
  • Nucleophosmin
  • Numatrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-79876AF350
Alexa Fluor 405 NBP2-79876AF405
Alexa Fluor 488 NBP2-79876AF488
Alexa Fluor 532 NBP2-79876AF532
Alexa Fluor 594 NBP2-79876AF594
Alexa Fluor 647 NBP2-79876AF647
Alexa Fluor 700 NBP2-79876AF700
Alexa Fluor 750 NBP2-79876AF750
Biotin NBP2-79876B
DyLight 350 NBP2-79876UV
DyLight 405 NBP2-79876V
DyLight 488 NBP2-79876G
DyLight 550 NBP2-79876R
DyLight 594 NBP2-79876DL594
DyLight 650 NBP2-79876C
DyLight 680 NBP2-79876FR
DyLight 755 NBP2-79876IR
FITC NBP2-79876F
HRP NBP2-79876H
Janelia Fluor 549 NBP2-79876JF549
Janelia Fluor 646 NBP2-79876JF646

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Bioinformatics

Gene Symbol NPM1